1. Syntax and Language: Mockito is a mocking framework for Java, while pytest is a testing framework for Python. Consequently, Mockito uses Java syntax and relies on Java-specific features, while pytest uses Python syntax and leverages Python-specific functionalities.

2. Integration with JUnit/Jupiter: Mockito integrates seamlessly with JUnit and JUnit 5 (Jupiter), which are popular unit testing frameworks for Java. On the other hand, pytest does not have a built-in integration with a specific testing framework. However, it can be used with other Python testing frameworks like unittest or doctest.

3. Fixture Management: In pytest, fixture management is a prominent feature. Fixtures in pytest are functions that provide and manage test resources and dependencies. On the contrary, Mockito does not have a built-in mechanism for managing fixtures, as it mainly focuses on mocking and stubbing objects.

4. Test Doubles and Mocking: Mockito focuses on the creation of test doubles, such as mock objects, stubs, and spies. It provides capabilities for flexible mocking, method verification, and behavior verification. In comparison, pytest supports mocking through libraries like unittest.mock, but it does not have in-built capabilities specifically tailored for test doubles.

5. Test Organization and Discovery: Pytest offers powerful test discovery mechanisms and provides various ways to organize tests, including test modules, test classes, and test functions. It allows test collection based on naming conventions and flexible test selection options. In contrast, Mockito does not have built-in test discovery capabilities and relies on external test runners like JUnit to organize and execute tests.

6. Community Support and Ecosystem: Both Mockito and pytest have active communities and extensive ecosystems of plugins, extensions, and integrations. However, Mockito's community and ecosystem primarily revolve around Java and the JVM, while pytest's community and ecosystem are centered around Python and its associated technologies.

In summary, Mockito is a Java-specific mocking framework tightly integrated with JUnit, focusing on providing test doubles and advanced mocking capabilities. On the other hand, pytest is a Python testing framework that embraces Python's syntax and offers a versatile testing experience with powerful test discovery, fixture management, and flexible test organization options.